| Title | Hydrogen-deuterium (h-d) exchange reaction of warfarin in d(2)o solution. | | Author(s) | Tode C, Takeuchi A, Iwakawa S, Tatsumi A, Sugiura M | | Institution | Kobe Pharmaceutical University. | | Source | Chem Pharm Bull (Tokyo) 2009 Jul; 57(7):653-6. | | Abstract | To prove the presence of a hydrogen-deuterium (H-D) exchange reaction, (1)H- and (13)C-NMR spectra of warfarin were measured in solvents containing D(2)O and H(2)O. In D(2)O or D(2)O/dimethyl sulfoxide (DMSO)-d(6) solvent, signal pattern changes were observed on H12 and H11 as well as 14 methyl protons over time while no changes were observed on H(2)O or H(2)O/DMSO-d(6) solvent. The observed changes in the solvents containing D(2)O were concluded to be caused by the H-D exchange reaction on H12, the process of CH(2)-->CHD-->CD(2). MS spectroscopy also confirmed these H-D exchanges. The kinetics of this reaction were analyzed as the successive reaction, and the mechanism was also proposed. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19571407 |
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