CE with a new electrochemiluminescent detection system for separation and detection of proteins labeled with tris(1,10-phenanthroline) ruthenium(II). Electrophoresis [Electrophoresis] Journal article | | Title | CE with a new electrochemiluminescent detection system for separation and detection of proteins labeled with tris(1,10-phenanthroline) ruthenium(II). | | Author(s) | Guo L, Qiu B, Xue L, Chen G | | Institution | Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety Department of Chemistry, Fuzhou University, Fuzhou, P. R. China. | | Source | Electrophoresis 2009 Jul 1. | | Abstract | In this article, a CE with a new electrochemiluminescent (ECL) detection system was developed. A microfluidic ECL detection cell with less than 0.5 muL dead volumes was developed and used as detector for this system. A hydrofluoric acid-etched porous joint was made at 8 mm from the outlet of the separation capillary to isolate the CE high voltage from ECL detection. The proposed CE-ECL system was applied for separation and detection of some proteins labeled with tris(1,10-phenanthroline) ruthenium(II). High efficiency ECL-enhanced reagent, tripropylamine, was infused to the detection cell as coreactant by a micro-infusion system to obtain maximum and stable ECL signal. The performance of this setup was illustrated by the analysis of tris(1,10-phenanthroline) ruthenium(II)-labeled proteins. The background electrolyte for protein detection was 20 mM Tris-CH(3)COOH with 2.0% m/m PVP at pH 4.0. Under the optimal conditions, the corresponding LOD were 2.2x10(-10) M for HSA, 4.4x10(-10) M for casein (alpha-S1) and 5.1x10(-10) M for cytochrome c. The proposed method was also successfully used for the trace analysis of albumin in human urine without any pretreatment. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19572322 |
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