| Title | Freezing equine semen: the effect of combinations of semen extenders and glycerol on post-thaw motility. | | Author(s) | Scherzer J, Fayrer-Hosken RA, Aceves M, Hurley DJ, Ray LE, Jones L, Heusner GL | | Institution | College of Veterinary Medicine, University of Georgia, Athens, GA, USA; jakobs@uga.edu. | | Source | Aust Vet J 2009 Jul; 87(7):275-279. | | Abstract | Objective We evaluated combinations of two commercial semen extenders and three concentrations of glycerol to determine the combination that yielded the highest post-thaw sperm motility. Design A randomised 2 x 3 block design was used. Procedure Semen was collected from four stallions (6 collections per stallion). The sample was diluted with either a dried skim-milk glucose extender (EZ Mixin Original Formula) or a chemically defined, milk-free diluent (INRA 96), and each was used in combination with 2%, 3% or 4% glycerol in standard commercial freezing medium. Sperm motility was assessed by microscopy in fresh and post-thaw semen. Results There was a significant difference between the two extenders in the motility of spermatozoa after cryopreservation (48.9% for INRA 96; 38.6% for EZ Mixin OF; P < 0.0001). Glycerol at 4% in freezing medium yielded the highest post-thaw motility, significantly better than 2% (P < 0.05). Three of four stallions had significantly higher post-thaw motility using INRA 96 relative to EZ Mixin OF (P < 0.01), and two of four stallions had significantly higher post-thaw motility using 4% glycerol (P < 0.05). The combination of INRA 96 and 4% glycerol in freezing medium gave the highest average post-thaw motility of 51.5%. Conclusion In this study, INRA 96 combined with 4% glycerol yielded an average recovery of progressively motile sperm consistently above the 35% target. Aust Vet J 2009;87:275-279. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19573151 |
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