Identification of genetic and phenotypic differences associated with prevalent and non-prevalent Salmonella Enteritidis phage types: analysis of variation in amino acid transport. Microbiology (Reading, England) [Microbiology] Journal article | | Title | Identification of genetic and phenotypic differences associated with prevalent and non-prevalent Salmonella Enteritidis phage types: analysis of variation in amino acid transport. | | Author(s) | Pan Z, Carter B, Núñez-García J, Abuoun M, Fookes M, Ivens A, Woodward MJ, Anjum MF | | Institution | Veterinary Laboratories Agency; | | Source | Microbiology 2009 Jul 2. | | Abstract | In this study differences at the genetic level of 37 S. Enteritidis strains from 5 phage types were compared using Comparative Genomic Hybridisation (CGH) to assess differences between phage types. There were approximately 400 genes that differentiated prevalent (PTs 4, 6, 8 13a) and sporadic (PT11) phage types of which 35 genes were unique to prevalent phage types, including 6 plasmid borne genes pefA,B,C,D, srgC and rck, and 4 chromosomal genes encoding for a putative amino acid transporter. Phenotype array studies also demonstrated that strains from prevalent phage types were less susceptible to urea stress and utilised L-histidine, L-glutamine, L-proline, L-aspartic-acid, gly-asn and gly-gln more efficiently than PT11 strains. Complementation of a PT11 strain with the transporter genes from PT4 resulted in a significant increase in utilization of the amino acids and reduced susceptibility to urea stress. In epithelial cell association assays, PT11 strains were less invasive than other prevalent phage types. Most strains from prevalent phage types were good biofilm former at 37 degrees C compared to 28 degrees C, whilst the converse was true for PT11 strains. Collectively, the results indicate genetic and corresponding phenotypic differences exist between the prevalent phage types 4, 6, 8,13a and non-prevalent phage type11 strains that are likely to provide a selective advantage for strains from the former phage types which could help them to enter the food chain and cause Salmonellosis. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19574306 |
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