Jmjd6 catalyses lysyl-hydroxylation of U2AF65, a protein associated with RNA splicing. Science (New York, N.Y.) [Science] Journal article

Title	Jmjd6 catalyses lysyl-hydroxylation of U2AF65, a protein associated with RNA splicing.
Author(s)	Webby CJ, Wolf A, Gromak N, Dreger M, Kramer H, Kessler B, Nielsen ML, Schmitz C, Butler DS, Yates JR, Delahunty CM, Hahn P, Lengeling A, Mann M, Proudfoot NJ, Schofield CJ, Böttger A
Institution	Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, Oxon OX1 3TA, UK.
Source	Science 2009 Jul 3; 325(5936):90-3.
MeSH	Alternative Splicing Amino Acid Sequence Biocatalysis Cell Line Chromatography, Liquid Hela Cells Humans Hydroxylation Lysine Molecular Sequence Data Nuclear Proteins Protein Processing, Post-Translational RNA, Small Interfering Receptors, Cell Surface Recombinant Proteins Ribonucleoproteins Tandem Mass Spectrometry Tropomyosin
Abstract	The finding that the metazoan hypoxic response is regulated by oxygen-dependent posttranslational hydroxylations, which regulate the activity and lifetime of hypoxia-inducible factor (HIF), has raised the question of whether other hydroxylases are involved in the regulation of gene expression. We reveal that the splicing factor U2 small nuclear ribonucleoprotein auxiliary factor 65-kilodalton subunit (U2AF65) undergoes posttranslational lysyl-5-hydroxylation catalyzed by the Fe(II) and 2-oxoglutarate-dependent dioxygenase Jumonji domain-6 protein (Jmjd6). Jmjd6 is a nuclear protein that has an important role in vertebrate development and is a human homolog of the HIF asparaginyl-hydroxylase. Jmjd6 is shown to change alternative RNA splicing of some, but not all, of the endogenous and reporter genes, supporting a specific role for Jmjd6 in the regulation of RNA splicing.
Language	eng
Pub Type(s)	Journal Article Research Support, Non-U.S. Gov't
PubMed ID	19574390

Advertise on this site.