| Title | An improved primary human nasal cell culture for the simultaneous determination of transepithelial transport and ciliary beat frequency. | | Author(s) | Mallants R, Vlaeminck V, Jorissen M, Augustijns P | | Institution | Laboratory for Pharmacotechnology and Biopharmacy, Katholieke Universiteit Leuven, Belgium. | | Source | J Pharm Pharmacol 2009 Jul; 61(7):883-90. | | Abstract | OBJECTIVES: The aim was to establish a preclinical in-vitro system of the nasal mucosa for the simultaneous evaluation of nasal absorption and effects on ciliary activity.
METHODS: Human nasal epithelial cells were grown in collagen-coated transport inserts with transparent polyethylene terephthalate membranes (3 mum). Transepithelial transport and ciliary beat frequency values were measured every 15 min for 1 h. KEY
FINDINGS: The apparent permeability coefficients (P(app)) for atenolol (mainly paracellular transport) and propranolol (transcellular transport) amounted to 0.1 +/- 0.1 and 23.7 +/- 0.6 x 10(-6) cm/s, respectively, illustrating that the system can be used to discriminate between high permeability and low permeability compounds. Transport of talinolol (substrate for the P-glycoprotein efflux carrier) did not reveal polarity (0.3 +/- 0.2 and 0.2 +/- 0.1 x 10(-6) cm/s for absorptive and secretory transport, respectively) and was not affected by verapamil (10 muM), suggesting the absence of P-glycoprotein in the nasal cell culture. No significant effects of atenolol, propranolol and talinolol on ciliary beat frequency were observed (98 +/- 20% of the control condition after 60 min). Chlorocresol significantly decreased the ciliary activity but this decrease was not accompanied by effects on the transepithelial transport of atenolol, propranolol and talinolol.
CONCLUSIONS: A new system was developed which offers possibilities as a fast screening tool for studying the potential of compounds for nasal drug administration, since permeability and a possible cilio-toxic effect can be assessed simultaneously. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19589230 |
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