Gordien JB, Pigneux A, Vigouroux S, Tabrizi R, Accoceberry I, Bernadou JM, Rouault A, Saux MC, Breilh D
Simultaneous determination of five systemic azoles in plasma by high-performance liquid chromatography with ultraviolet detection. [JOURNAL ARTICLE]
J Pharm Biomed Anal 2009 Jun 24.
A simple, specific and automatable HPLC assay was developed for a simultaneous determination of systemic azoles (fluconazole, posaconazole, voriconazole, itraconazole and its metabolite hydroxyl-itraconazole, and ketoconazole) in plasma. The major advantage of this assay was sample preparation by a fully automatable solid phase extraction with Varian Plexa cartridges. C6-phenyl column was used for chromatographic separation, and UV detection was set at a wavelength of 260nm. Linezolid was used as an internal standard. The assay was specific and linear over the concentration range of 0.05 to 40mug/ml excepted for fluconazole which was between 0.05 and 100mug/ml, and itraconazole between 0.1 and 40mug/ml. Validation data for accuracy and precision for intra- and inter-day were good and satisfied FDA's guidance: CV between 0.24% and 11.66% and accuracy between 93.8% and 108.7% for all molecules. This assay was applied to therapeutic drug monitoring on patients hospitalized in intensive care and onco-hematologic units.
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