| Title | Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells. | | Author(s) | Huang YH, Sun MJ, Jiang M, Fu BY | | Institution | Department of Gastroenterology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China jiangminhyh@hotmail.com. | | Source | Eur J Histochem 2009 Apr-Jun; 53(2):81-6. | | Abstract | We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PPsecreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19683981 |
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