| Title | A convenient method for synthesis of glyconanoparticles for colorimetric measuring carbohydrate-protein interactions. | | Author(s) | Chuang YJ, Zhou X, Pan Z, Turchi C | | Institution | Faculty of Engineering, University of Georgia, Athens, GA 30602, USA. | | Source | Biochem Biophys Res Commun 2009 Aug 18. | | Abstract | Carbohydrate functionalized nanoparticles, i.e., the glyconanoparticles, have wide application ranging from studies of carbohydrate-protein interactions, in vivo cell imaging, biolabeling, etc. Currently reported methods for preparation of glyconanoaprticles require multi-step modifications of carbohydrates moieties to conjugate to nanoparticle surface. However, the required synthetic manipulations are difficult and time consuming. We report herewith a simple and versatile method for preparing glyconanoparticles. This method is based on the utilization of clean and convenient microwave irradiation energy for one-step, site-specific conjugation of unmodified carbohydrates onto hydrazide-functionalized Au nanoparticles. A colorimetric assay that utilizes the ensemble of gold glyconanoparticles and Concanavalin A (ConA) was also presented. This feasible assay system was developed to analyze multivalent interactions and to determine the dissociation constant (K(d)) for five kind of Au glyconanoparticles with lectin. Surface plasmon changes of the Au glyconanparticles as a function of lectin-carbohydrate interactions were measured and the dissociation constants were determined based on non-linear curve fitting. The strength of the interaction of carbohydrates with ConA was found to be as follows: Maltose > Mannose > Glucose > Lactose > MAN5. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19698698 |
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