| Title | Electrophoretically mediated microanalysis technique as a tool for the rapid screening of novel acetylcholinesterase inhibitors. | | Author(s) | Bajda M, Wieckowska A, Malawska B | | Institution | Jagiellonian University Medical College, Department of Physicochemical Drug Analysis, Chair of Pharmaceutical Chemistry, Medyczna 9, 30-688 Kraków, Poland. | | Source | Acta Pol Pharm 2009 Jul-Aug; 66(4):357-62. | | Abstract | An electrophoretically mediated microanalysis technique was developed for the rapid screening of acetylcholinesterase inhibitors. Activity of four original compounds, N-benzylpiperidine, carbamoyloxyphenyl derivatives and reference inhibitor--tacrine was determined and compared with the data obtained from Ellman's assay. Percentage of enzyme inhibition detected at inhibitor concentration of 3.33.10(-4) M was between 0 and 78% and was the highest for tacrine. The comparison of data obtained from the EMMA assay and Ellman's test proved inhibitory activity of novel compounds. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19702166 |
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