Upadhyay NK, Kumar R, Mandotra SK, Meena RN, Siddiqui MS, Sawhney RC, Gupta A
Safety and healing efficacy of Sea buckthorn (Hippophae rhamnoides L.) seed oil on burn wounds in rats. [JOURNAL ARTICLE]
Food Chem Toxicol 2009 Feb 10.
The present investigation was undertaken to determine the safety and efficacy of supercritical CO(2)-extracted Hippophae rhamnoides L. (Sea buckthorn) seed oil on burn wound model. SBT seed oil was co-administered by two routes at a dose of 2.5ml/kg body weight (p.o.) and 200mul (topical) for 7days on experimental burn wounds in rats. The SBT seed oil augmented the wound healing process as indicated by significant increase in wound contraction, hydroxyproline, hexosamine, DNA and total protein contents in comparison to control and reference control treated with silver sulfadiazine ointment. Histopathological findings further confirmed the healing potential of SBT seed oil. SBT seed oil treatment up-regulated the expression of matrix metalloproteinases (MMP-2 and 9), collagen type-III and VEGF in granulation tissue. It was observed that SBT seed oil also possesses antioxidant properties as evidenced by significant increase in reduced glutathione (GSH) level and reduced production of reactive oxygen species (ROS) in wound granulation tissue. In acute and sub-acute oral toxicity studies, no adverse effects were observed in any of the groups administered with SBT seed oil. These results suggest that the supercritical CO(2)-extracted Sea buckthorn seed oil possesses significant wound healing activity and have no associated toxicity or side effects.
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