Evaluation of different methods for assessment of insulin sensitivity in Gottingen minipigs - introduction of a new simpler method. American journal of physiology. Regulatory, integrative and comparative physiology [Am J Physiol Regul Integr Comp Physiol] Journal article | | Title | Evaluation of different methods for assessment of insulin sensitivity in Gottingen minipigs - introduction of a new simpler method. | | Author(s) | Christoffersen BO, Ribel U, Raun K, Golozoubova V, Pacini G | | Institution | Novo Nordisk A/S. | | Source | Am J Physiol Regul Integr Comp Physiol 2009 Aug 26. | | Abstract | The use of animal models in diabetes research requires reliable tests for evaluation of insulin sensitivity and beta cell function. Minipigs are being increasingly used in metabolic research, and the aim of this study was to compare different tests and indices for evaluation of insulin sensitivity and beta cell function in Göttingen minipigs. Hyperinsulinemic, isoglycemic clamp, intravenous (IVGTT) and oral (OGTT) glucose tolerance tests and modified insulin tolerance test were performed in minipigs fed either low or high energy diet. Furthermore, the reproducibility of IVGTT derived parameters was assessed. Previously described insulin sensitivity indices (steady state glucose infusion rate/glucose concentration/insulin concentration from clamp (M/G/I); oral glucose insulin sensitivity (OGIS) and ISIcomp from OGTT; SI from minimal model analysis of IVGTT and quantitative insulin sensitivity check index from fasting values) were calculated together with an insulin sensitivity index from the modified insulin tolerance test, ISIITT, and a new simple index, S2, derived from the first 30 min of the IVGTT. Beta cell function was assessed from IVGTT and OGTT. Reproducibility of the IVGTT indices was calculated as median intraindividual CV%. M/G/I correlated significantly only with the new index, S2 (p<0.05, r=0.54). S2 furthermore correlated with SI (p<0.001, r=0.81), ISIITT (p<0.001, r=0.57) and the two indices from OGTT, ISIcomp(p<0.001, r=0.78) and OGIS (p<0.05, r=0.48). No correlation was found between beta cell function indices from OGTT and IVGTT. The median CV% of the new S2 index was 13. In conclusion, the new simple index of insulin sensitivity, S2, revealed to be useful for evaluation of insulin sensitivity in Göttingen minipigs. Key words: glucose tolerance, beta cell function, IVGTT, OGTT. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19710388 |
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