Enantioselective analysis of R- and S-propafenone in plasma by HPLC applying column switching and liquid-liquid extraction. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | Enantioselective analysis of R- and S-propafenone in plasma by HPLC applying column switching and liquid-liquid extraction. | | Author(s) | Lamprecht G, Stoschitzky K | | Institution | University of Vienna, Institute of Analytical Chemistry, Währingerstrasse 38, A-1090 Vienna, Austria. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2009 Aug 21. | | Abstract | Two HPLC methods are described for the enantioselective analysis of R- and S-propafenone in plasma. In a column switching approach, centrifuged plasma was injected onto a silica-based strong acid cation-exchanger and the fraction containing propafenone was switched on-line onto an enantioselective Chiralcel column for separation of the enantiomers. In another approach, propafenone was extracted from plasma by liquid-liquid extraction at pH 11.4. The extracted components were transferred into aqueous medium and separated on a Chiralcel ODR. Both methods were validated and showed comparable performance. Within-day and between-day precision was better than 5% for both methods. Linear calibration functions were obtained (r(2)>0.999), and the limit of detection for each enantiomer was 0.2mug/mL for column switching and 0.55mug/mL for liquid-liquid extraction. The analysis methods were applied for the determination of the effect of physical exercise on the enantiomeric ratio of R- and S-propafenone in plasma of healthy volunteers. During exercise, the concentration of both enantiomers reached a maximum, followed by a significant decrease during recovery. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19736049 |
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