| Title | Glutathione Disulfide and S-Nitrosoglutathione Detoxification by Mycobacteriumtuberculosis Thioredoxin System. | | Author(s) | Attarian R, Bennie C, Bach H, Av-Gay Y | | Institution | Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, British Columbia, V5Z 3J5, Canada. | | Source | FEBS Lett 2009 Sep 5. | | Abstract | Mycobacterium tuberculosis resides within alveolar macrophages. These phagocytes produce reactive nitrogen and oxygen intermediates to combat the invading pathogens. The macrophage glutathione (GSH) pool reduces nitric oxide (NO) to S-nitrosoglutathione (GSNO). Both glutathione disulphide (GSSG) and GSNO possess mycobactericidal activities in vitro. In this study we demonstrate that M. tuberculosis thioredoxin system, comprises of thioredoxin reductase B2 and thioredoxin C reduces the oxidized form of the intracellular mycothiol (MSSM) and is able to efficiently reduce GSSG and GSNO in vitro. Our study suggests that the thioredoxin system provide a general reduction mechanism to cope with oxidative stress associated with the microbe's metabolism as well as to detoxify xenobiotics produced by the host. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19737561 |
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