| Title | Comparison of clonal relatedness and antimicrobial susceptibility of fecal Escherichia coli from healthy dogs and their owners. | | Author(s) | Stenske KA, Bemis DA, Gillespie BE, D'Souza DH, Oliver SP, Draughon FA, Matteson KJ, Bartges JW | | Source | J Am Vet Med Assoc 2009 Sep 15; 235(6):754. | | Abstract | Objective-To determine prevalence of within-household sharing of fecal Escherichia coli between dogs and their owners on the basis of pulsed-field gel electrophoresis (PFGE), compare antimicrobial susceptibility between isolates from dogs and their owners, and evaluate epidemiologic features of cross-species sharing by use of a questionnaire. Sample Population-61 healthy dog-owner pairs and 30 healthy control humans. Procedures-3 fecal E coli colonies were isolated from each participant; PFGE profiles were used to establish relatedness among bacterial isolates. Susceptibility to 17 antimicrobials was determined via disk diffusion. A questionnaire was used to evaluate signalment, previous antimicrobial therapy, hygiene, and relationship with dog. Results-A wide array of PFGE profiles was observed in E coli isolates from all participants. Within-household sharing occurred with 9.8% prevalence, and across-household sharing occurred with 0.3% prevalence. No behaviors were associated with increased clonal sharing between dog and owner. No differences were found in susceptibility results between dog-owner pairs. Control isolates were more likely than canine isolates to be resistant to ampicillin and trimethoprim-sulfamethoxazole. Owners and control humans carried more multidrug-resistant E coli than did dogs. Conclusions and Clinical Relevance-Within-household sharing of E coli was detected more commonly than across-household sharing, but both direct contact and environmental reservoirs may be routes of cross-species sharing of bacteria and genes for resistance. Cross-species bacterial sharing is a potential public health concern, and good hygiene is recommended. (Am J Vet Res 2009;70:1108-1116). | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19751175 |
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