| Title | Newly developed tissue-engineered material for reconstruction of vascular wall without cell seeding. | | Author(s) | Takahashi H, Yokota T, Uchimura E, Miyagawa S, Ota T, Torikai K, Saito A, Hirakawa K, Kitabayashi K, Okada K, Sawa Y, Okita Y | | Institution | Department of Surgery, Division of Cardiovascular Surgery, Kobe University Graduate School of Medicine, Kobe, Japan. | | Source | Ann Thorac Surg 2009 Oct; 88(4):1269-76. | | MeSH | Absorbable Implants
Animals
Blood Vessel Prosthesis
Disease Models, Animal
Dogs
Follow-Up Studies
Immunohistochemistry
Microscopy, Electron, Scanning
Muscle, Smooth, Vascular
Prosthesis Design
Pulmonary Artery
Tensile Strength
Tissue Engineering
| | Abstract | BACKGROUND: We have developed a tissue-engineered patch for cardiovascular repair. Tissue-engineered patches facilitated site-specific in situ recellularization and required no pretreatment with cell seeding. This study evaluated the patches implanted into canine pulmonary arteries.
METHODS: Tissue-engineered patches are biodegradable sheets woven with double-layer fibers. The fiber is composed of polyglycolic acid and poly-L-lactic acid, and compounding collagen microsponges. The patches (20- x 25-mm) were implanted into the canine pulmonary arterial trunks. At 1, 2, and 6 months after implantation (n = 4), they were explanted and characterized by histologic and biochemical analyses. Commercially available patches served as the control. No anticoagulant therapy was administered postoperatively.
RESULTS: No aneurysm or thrombus was present within the patch area in all groups. The remodeled tissue predominantly consisted of elastic and collagen fibers, and the endoluminal surface was covered with a monolayer of endothelial cells and multilayers of smooth muscle cells beneath the endothelial layer. The elastic and collagen fibers and smooth muscle cells kept increasing with a maximum at 6 months, while a monolayer of endothelial cells was preserved. The expression levels of messenger RNA of several growth factors in the tissue-engineered patches were higher than those of native tissue at 1 and 2 months and decreased to normal level at 6 months. No regenerated tissue was found on the endoluminal surface in the control group.
CONCLUSIONS: The novel tissue-engineered patches showed in situ repopulation of host cells without prior ex vivo cell seeding. This is promising material for repair of the cardiovascular system. | | Language | eng | | Pub Type(s) | Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 19766820 |
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