Sinturel F, Pellegrini O, Xiang S, Tong L, Condon C, Bénard L
Real-time fluorescence detection of exoribonucleases. [JOURNAL ARTICLE]
RNA 2009 Sep 18.
The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA-DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx). This RT-FeDEx assay provides a new strategy for the isolation, purification, and assay of known and unknown exoribonucleases.
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