| Title | Real-time fluorescence detection of exoribonucleases. | | Author(s) | Sinturel F, Pellegrini O, Xiang S, Tong L, Condon C, Bénard L | | Institution | Institut de Biologie Physico-Chimique, CNRS UPR 9073, Université de Paris 7-Denis Diderot, 75005 Paris, France. | | Source | RNA 2009 Sep 18. | | Abstract | The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA-DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx). This RT-FeDEx assay provides a new strategy for the isolation, purification, and assay of known and unknown exoribonucleases. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19767421 |
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