Regulation of gonadotropin-releasing hormone secretion by kisspeptin/dynorphin/neurokinin B neurons in the arcuate nucleus of the mouse. The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] Journal article | | Title | Regulation of gonadotropin-releasing hormone secretion by kisspeptin/dynorphin/neurokinin B neurons in the arcuate nucleus of the mouse. | | Author(s) | Navarro VM, Gottsch ML, Chavkin C, Okamura H, Clifton DK, Steiner RA | | Institution | Department of Physiology and Biophysics, University of Washington, Seattle, 98195, USA. | | Source | J Neurosci 2009 Sep 23; 29(38):11859-66. | | MeSH | Animals
Arcuate Nucleus
Dynorphins
Estradiol
Female
Gonadotropin-Releasing Hormone
In Situ Hybridization
Luteinizing Hormone
Mice
Mice, Inbred C57BL
Mice, Knockout
Models, Neurological
Neurokinin B
Neurons
Ovariectomy
Protein Precursors
RNA, Messenger
Receptors, Neurokinin-3
Receptors, Opioid
Signal Transduction
Tumor Suppressor Proteins
| | Abstract | Kisspeptin is encoded by the Kiss1 gene, and kisspeptin signaling plays a critical role in reproduction. In rodents, kisspeptin neurons in the arcuate nucleus (Arc) provide tonic drive to gonadotropin-releasing hormone (GnRH) neurons, which in turn supports basal luteinizing hormone (LH) secretion. Our objectives were to determine whether preprodynorphin (Dyn) and neurokinin B (NKB) are coexpressed in Kiss1 neurons in the mouse and to evaluate its physiological significance. Using in situ hybridization, we found that Kiss1 neurons in the Arc of female mice not only express the Dyn and NKB genes but also the NKB receptor gene (NK3) and the Dyn receptor [the kappa opioid receptor (KOR)] gene. We also found that expression of the Dyn, NKB, KOR, and NK3 in the Arc are inhibited by estradiol, as has been established for Kiss1, and confirmed that Dyn and NKB inhibit LH secretion. Moreover, using Dyn and KOR knock-out mice, we found that long-term disruption of Dyn/KOR signaling compromises the rise of LH after ovariectomy. We propose a model whereby NKB and dynorphin act autosynaptically on kisspeptin neurons in the Arc to synchronize and shape the pulsatile secretion of kisspeptin and drive the release of GnRH from fibers in the median eminence. | | Language | eng | | Pub Type(s) | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
| | PubMed ID | 19776272 |
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