| Title | Effect of Mg2+ on neural activity of rat cortical and hippocampal neurons in vitro. | | Author(s) | Furukawa Y, Kasai N, Torimitsu K | | Institution | NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa 243-0198, Japan. yuri@will.brl.ntt.co.jp | | Source | Magnes Res 2009 Sep; 22(3):174S-181S. | | Abstract | Mg2+ plays an important role in biological functions, similar to that of Ca2+. In terms of neural activity, it is well known that Mg2+ blocks the NMDA receptor. However, the relationship between Mg2+ and neural function has not been well understood. We have investigated the effect of low extracellular Mg2+ concentration ([Mg2+]o) on neural activity in rat cortical and hippocampal neurons by using microelectrode array (MEA) measurements and glutamate measurements, with an enzyme modified MEA-based multi-array sensor. In this study, we investigated the effects of low [Mg2+]o on intracellular Ca2+ concentration ([Ca2+]i) using a confocal laser microscope and a flow cytometer with a fluorescence probe. The results indicate that low [Mg2+]o has an effect on neural activity. The responses of cortical and hippocampal neurons to low [Mg2+]o differed in the developmental period. The results suggest that hippocampal neurons are more sensitive to [Mg2+ than cortical neurons. The glutamate receptor distributions in the cortex and hippocampus may be different. Further investigation is required to understand the mechanisms of the Mg2+ effect on neural activity. | | Language | eng | | Pub Type(s) | Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 19780405 |
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