| Title | Anti-vascular endothelial growth factor activity in the bevacizumab and triamcinolone acetonide combination for intravitreal use. | | Author(s) | Giammaria D, Cinque B, Di Lodovico D, Savastano MC, Cifone MG, Spadea L | | Institution | Department of Surgical Sciences, Eye Clinic, University of L'Aquila - Italy. | | Source | Eur J Ophthalmol 2009 September-October; 19(5):842-847. | | Abstract | Purpose. To find out if the combination for intravitreal use of the antibody bevacizumab (AvastinTM; Genentech, Inc., San Francisco, CA) and triamcinolone acetonide (TA) (Kenacort®; Bristol-Myers Squibb, Anagni, Italy) could affect over time the anti -vascular endothelial growth factor (VEGF) activity of bevacizumab. Methods. Two different combined preparations were obtained, drawing up together 1.25 mg/0.05 mL of bevacizumab and 2 mg/0.05 mL (B+TA2mg) or 4 mg/0.05 mL (B+TA4mg) of TA into insulin syringes with 29-G needle. Control preparations were obtained with bevacizumab and an injectable solution (B). The syringes were stored refrigerated at 4 degrees C. The bevacizumab concentration was measured, through its binding to VEGF-165 isoform, at 48 hours and at 1 week.
Results. No preparations showed statistically significant changes in bevacizumab concentration with time (p=0.74 for B+T2mg, p=0.92 for B+T4mg, p=0.57 for B). The B+TA2mg preparations showed a larger percentage of degradation of bevacizumab than the B+TA4mg preparations (28.4% versus 17.6% at 48 hours; 26.4% versus 18% at 1 week). The B control preparations showed the lowest drug degradation: 9.6% at 48 hours and 14.8% at 1 week.
Conclusions. After storage at 4 degrees C for 48 hours and 1 week, the combined preparations showed a larger reduction in bevacizumab concentration than the control preparations. No significant change was observed with the length of storage. The preparations obtained mixing 4 mg/0.05 mL of TA and 1.25 mg/0.05 mL of bevacizumab maintained the highest anti-VEGF activity over time. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19787607 |
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