Resch M, Resch B, Csizmazia E, Imre L, Nemeth J, Revesz P, Csanyi E
Permeability of human amniotic membrane to ofloxacin in vitro. [JOURNAL ARTICLE]
Invest Ophthalmol Vis Sci 2009 Sep 24.
Purpose: The aim of our study was to develop a model to investigate permeability of amniotic membrane (AM) to ofloxacin eye drops, a widely used topical antibiotic in ocular surface disease after AM transplantation.
Methods: AM pieces on cellulose acetate filter membranes were mounted in vertical Franz-diffusion cell system equipped with autosampler. In vitro release of 300 mg of 3% commercially available ofloxacin ophthalmic solution was determined by quantitative absorbance measurement carried out with a UV spectrophotometer (wavelength 287nm). Freshly prepared and cryopreserved AMs were compared. Porafil filter membranes without AM served as positive controls.
Results. Ofloxacin was detectable in the acceptor phase 1 minute after instillation, and a gradual increase of concentration could be detected in a period of 90 minutes in all groups. At 30 minutes 3.35+/-2.23% of ofloxacin penetrated through freshly prepared AM, 4.35+/-1.8 through cryopreserved AM compared to 17.52+/-3.91 filter membrane alone. At 90 minutes penetration rates of ofloxacin were 5.04+/-1.11%, 5.26+/-3.21%, 27.91+/-3.05%, respectively. Difference (p>0.05 t-test) was not significant between freshly prepared and cryopreserved AMs. Compared to control, both membranes showed significant difference (p<0.05 t-test) at all timepoints.
Conclusion: The in vitro model of Franz-diffusion cell system was found to be applicable for drug permeability studies of human amniotic membranes to water based solutions. The filter membrane and AM were permeable to water based solution of ofloxacin. Significant barrier function of AM could be measured in ofloxacin permeability. Cryopreservation did not influence permeability of AM.
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