Determination of paromomycin residues in turkey tissues by liquid chromatography/mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | Determination of paromomycin residues in turkey tissues by liquid chromatography/mass spectrometry. | | Author(s) | Róna K, Klausz G, Keller E, Szakay M, Laczay P, Shem-Tov M, Székely-Körmöczy P | | Institution | Semmelweis University, Department of Forensic and Insurance Medicine, Ulloi út 93, 1091 Budapest, Hungary. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2009 Sep 22. | | Abstract | A liquid chromatography-electrospray-mass spectrometry method has been developed and validated for the determination of paromomycin in turkey muscle, liver and kidney, using kanamycin as internal standard. The method consists of solid-phase extractions on mixed-mode columns. The chromatographic separation was carried out on a C(18) column using binary gradient elution containing acetonitrile and 5mM pentafluoropropionic-acid in water. The method was evaluated for specificity, linearity, recovery, accuracy, limit of detection, limit of quantification, intra- and inter-day repeatability, and stability. It was proven to be selective, linear, precise and accurate over the concentration range tested (0.5xMRL-2xMRL for each tissue) with correlation coefficients >0.990. The method was successfully used for the residue determination of PARO from edible tissues of turkeys. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19801206 |
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