"Glycoprofiling bifidobacterial consumption of galacto-oligosaccharides by mass spectrometry reveals strain specific, preferential consumption of glycans" Applied and environmental microbiology [Appl Environ Microbiol] Journal article | | Title | "Glycoprofiling bifidobacterial consumption of galacto-oligosaccharides by mass spectrometry reveals strain specific, preferential consumption of glycans" | | Author(s) | Barboza M, Sela DA, Pirim C, Locascio RG, Freeman SL, German JB, Mills DA, Lebrilla CB | | Institution | Department of Chemistry, Microbiology Graduate Group, Department of Viticulture and Enology, Robert Mondavi Institute for Wine and Food Science, Department of Food Science and Technology, Robert Mondavi Institute for Wine and Food Science, and School of Medicine, University of California, Davis, One Shields Avenue, Davis, California 95616. | | Source | Appl Environ Microbiol 2009 Oct 2. | | Abstract | Galacto-oligosaccharides (GOS) are versatile food ingredients that possess prebiotic properties. However, at present there is a lack of precise analytical methods available to demonstrate specific GOS consumption by bifidobacteria. To better understand the role of GOS as prebiotics, purified GOS (pGOS) free of disaccharide- and monosaccharides was prepared and used in bacterial fermentation experiments. Growth curves showed that all bifidobacteria assayed utilized and grew on pGOS preparations. We applied a novel approach using mass spectrometry by employing matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance (MALDI-FTICR) to determine the composition of oligosaccharides in GOS syrup preparations. MALDI-FTICR analysis of spent fermentation media demonstrated the preferential consumption of select pGOS species by different bifidobacteria. The approach described here demonstrates that MALDI-FTICR is a rapid-throughput tool for comprehensive profiling of oligosaccharides within GOS mixtures. In addition, the selective consumption of certain GOS species by different bifidobacteria suggests a means for targeting prebiotics to enrich select bifidobacterial species. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19801485 |
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