Overproduction of the Pro-Apoptotic Molecule, Programmed Cell Death 5, in Toxoplasma gondii Leads to Increased Apoptosis of Host Macrophages. The Journal of veterinary medical science / the Japanese Society of Veterinary Science [J Vet Med Sci] Journal article | | Title | Overproduction of the Pro-Apoptotic Molecule, Programmed Cell Death 5, in Toxoplasma gondii Leads to Increased Apoptosis of Host Macrophages. | | Author(s) | Bannai H, Nishikawa Y, Ibrahim HM, Yamada K, Kawase O, Watanabe J, Sugimoto C, Xuan X | | Institution | National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine. | | Source | J Vet Med Sci 2009 Sep; 71(9):1183-9. | | Abstract | We established a recombinant strain of Toxoplasma gondii that overexpressed programmed cell death 5 (TgPDCD5), in order to evaluate the role of endogenous TgPDCD5 in macrophage apoptosis during T. gondii infection. Immunofluorescence microscopy revealed that overproduced TgPDCD5 with a hemagglutinin tag was localized in the cytosol, which was consistent with the localization of endogenous TgPDCD5. The induced TgPDCD5-HA was recognized as an additional band by Western blot analysis, indicating successful overexpression of TgPDCD5. Secretion and release of TgPDCD5 by the parasite was also up-regulated in a time-dependent manner, which reflected its overproduction. Apoptosis due to parasite infection and interferon-gamma treatment was significantly up-regulated by the overexpression of TgPDCD5. These results suggest that endogenous TgPDCD5 plays a role in macrophage apoptosis during T. gondii infection. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19801898 |
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