KATP channel mediates the zinc switch-off signal for glucagon response during glucose deprivation. Diabetes [Diabetes] Journal article

Objective- The intraislet insulin hypothesis proposes that glucagon secretion during hypoglycemia is triggered by a decrease in intraislet insulin secretion. A more recent hypothesis based on in vivo data from hypoglycemic rats is that it is the decrease in zinc co-secreted with insulin from beta-cells, rather than the decrease in insulin itself, that signals glucagon secretion from alpha-cells during hypoglycemia. These studies were designed to determine whether closure of the alpha-cell K(ATP) channel is the mechanism through which the zinc switch-off signal triggers glucagon secretion during glucose deprivation. Research Design and Methods - All studies were performed using perifused isolated islets. Results In control experiments, the expected glucagon response to an endogenous insulin switch-off signal during glucose deprivation was observed in wild type mouse islets. In experiments with streptozotocin-treated wild type islets, a glucagon response to an exogenous zinc switch-off signal was observed during glucose deprivation. However, this glucagon response to the zinc switch-off signal during glucose deprivation was not seen in the presence of nifedipine, diazoxide, or tolbutamide, or if K(ATP) channel knockout mouse islets were used. All islets had intact glucagon responses to epinephrine. Conclusions - These data demonstrate that closure of K(ATP) channels and consequent opening of calcium channels is the mechanism through which the zinc switch-off signal triggers glucagon secretion during glucose deprivation.

Diabetes [Diabetes]