Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science (New York, N.Y.) [Science] Journal article | | Title | Comprehensive mapping of long-range interactions reveals folding principles of the human genome. | | Author(s) | Lieberman-Aiden E, van Berkum NL, Williams L, Imakaev M, Ragoczy T, Telling A, Amit I, Lajoie BR, Sabo PJ, Dorschner MO, Sandstrom R, Bernstein B, Bender MA, Groudine M, Gnirke A, Stamatoyannopoulos J, Mirny LA, Lander ES, Dekker J | | Institution | Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), MA 02139, USA. | | Source | Science 2009 Oct 9; 326(5950):289-93. | | MeSH | Biotin
Cell Line, Transformed
Cell Nucleus
Chromatin
Chromatin Immunoprecipitation
Chromosomes, Human
Computational Biology
DNA
Gene Library
Genome, Human
Humans
In Situ Hybridization, Fluorescence
Models, Molecular
Monte Carlo Method
Nucleic Acid Conformation
Principal Component Analysis
Protein Conformation
Sequence Analysis, DNA
| | Abstract | We describe Hi-C, a method that probes the three-dimensional architecture of whole genomes by coupling proximity-based ligation with massively parallel sequencing. We constructed spatial proximity maps of the human genome with Hi-C at a resolution of 1 megabase. These maps confirm the presence of chromosome territories and the spatial proximity of small, gene-rich chromosomes. We identified an additional level of genome organization that is characterized by the spatial segregation of open and closed chromatin to form two genome-wide compartments. At the megabase scale, the chromatin conformation is consistent with a fractal globule, a knot-free, polymer conformation that enables maximally dense packing while preserving the ability to easily fold and unfold any genomic locus. The fractal globule is distinct from the more commonly used globular equilibrium model. Our results demonstrate the power of Hi-C to map the dynamic conformations of whole genomes. | | Language | eng | | Pub Type(s) | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
| | PubMed ID | 19815776 |
|
|
| |
Advertise on this site.
| | |
|
