Xiao Y, Zahariou G, Sanakis Y, Liu P
IspG Enzyme Activity in the Deoxyxylulose Phosphate Pathway: Roles of the Iron-Sulfur Cluster. [JOURNAL ARTICLE]
Biochemistry 2009 Oct 12.
IspG is a [4Fe-4S] cluster containing protein and the [4Fe-4S]1+ species is proposed to be the catalytically relevant species. However, attempts reported in the literature failed to detect the [4Fe-4S]1+ species. In this study, using a potent reduction system, we have successfully detected the [4Fe-4S]1+ species with X-band EPR spectroscopy. In addition, we have improved the E. coli IspG activity to 550 nmolmin-1mg-1, which is 20-fold greater than that of the NADPH-Fpr-FldA system in the literature.
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