| Title | Estrogen and selective estrogen receptor modulators regulate vascular endothelial growth factor and soluble vascular endothelial growth factor receptor 1 in human endometrial stromal cells. | | Author(s) | Okada H, Tsutsumi A, Imai M, Nakajima T, Yasuda K, Kanzaki H | | Institution | Department of Obstetrics and Gynecology, Kansai Medical University, Osaka, Japan. | | Source | Fertil Steril 2009 Oct 12. | | Abstract | OBJECTIVE: To determine whether 17beta-estradiol (E(2)) and selective estrogen receptor modulators can regulate vascular endothelial growth factor (VEGF) and soluble VEGF receptor 1 (sVEGFR-1) as a VEGF antagonist in human endometrial stromal cells (ESCs).
DESIGN: In vitro experiment.
SETTING: Research laboratory at Kansai Medical University.
PATIENT(S): Sixteen patients undergoing hysterectomy for benign reasons.
INTERVENTION(S): The ESCs were cultured with E(2), 4-hydroxytamoxifen (OHT), and raloxifene.
MAIN OUTCOME MEASURE(S): The VEGF and sVEGFR-1 messenger RNA (mRNA) levels in ESCs were determined using quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Free (unbound) VEGF and sVEGFR-1 protein levels from ESCs were measured using ELISA kits.
RESULT(S): The E(2) significantly induced VEGF mRNA levels, whereas E2 caused a significant decrease in sVEGFR-1 messenger RNA (mRNA) levels. The E(2) or OHT significantly increased the VEGF production levels and attenuated the sVEGFR-1 production compared with control, but raloxifene had no significant effect. The decrease in levels of free VEGF was proportional to the increase in sVEGFR-1 levels in the culture media of ESCs.
CONCLUSION(S): The E(2) or OHT stimulates VEGF production and concurrently attenuates sVEGFR-1 production in ESCs. This consequential increase in VEGF:sVEGFR-1 ratio might enhance the biological effects of VEGF on the angiogenic environment in human endometrium. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19828145 |
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