Pyrazinamide resistance in multidrug-resistant Mycobacterium tuberculosis isolates in Japan. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] Journal article

Abstract Thirty-six multidrug-resistant (MDR) Mycobacterium tuberculosis isolates collected in Japan were examined for pyrazinamide (PZA) -susceptibility, pyrazinamidase activity, the pncA sequencing, and a hybridization-based line probe assay (LiPA) which was used to detect pncA mutations for the rapid detection of in PZA-resistant M. tuberculosis. Of the isolates, 19 (53%) showed PZA resistance. All PZA-resistant isolates had no pyrazinamidase activity and at least one mutation in pncA. Among the pncA mutations, 11 were not previously reported. The results of the LiPA were fully consistent with the DNA sequencing results. A majority of MDR M. tuberculosis isolates in Japan had resistant to PZA.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect]