| Title | Pyrazinamide resistance in multidrug-resistant Mycobacterium tuberculosis isolates in Japan. | | Author(s) | Ando H, Mitarai S, Kondo Y, Suetake T, Sekiguchi JI, Kato S, Mori T, Kirikae T | | Institution | Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan. | | Source | Clin Microbiol Infect 2009 Oct 14. | | Abstract | Abstract Thirty-six multidrug-resistant (MDR) Mycobacterium tuberculosis isolates collected in Japan were examined for pyrazinamide (PZA) -susceptibility, pyrazinamidase activity, the pncA sequencing, and a hybridization-based line probe assay (LiPA) which was used to detect pncA mutations for the rapid detection of in PZA-resistant M. tuberculosis. Of the isolates, 19 (53%) showed PZA resistance. All PZA-resistant isolates had no pyrazinamidase activity and at least one mutation in pncA. Among the pncA mutations, 11 were not previously reported. The results of the LiPA were fully consistent with the DNA sequencing results. A majority of MDR M. tuberculosis isolates in Japan had resistant to PZA. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19832709 |
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