Li S, Liu G, Jia J, Miao Y, Gu S, Miao P, Shi X, Wang Y, Yu C
Therapeutic monitoring of serum digoxin for patients with heart failure using a rapid LC-MS/MS method. [JOURNAL ARTICLE]
Clin Biochem 2009 Oct 12.
Objective: Here we develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of digoxin in serum. Design and method: The serum samples were extracted with methyl tert-butyl ether using an isotope-labeled digoxin-d3 as internal standard. The analyte was separated on a reverse phase Capcell C18 column and detected in positive electrospray ionization multiple reaction monitoring mass spectrometry.
Results: The chromatographic analysis was carried out within 3 min, but the complete analysis took longer because of the liquid-liquid extraction. The lower limit of quantification was 0.1 ng/mL for digoxin. The intra-and inter-batch precisions were less than 12%, and the bias ranged from-9.1% to 10.7%. The external quality assessment (EQA) results obtained with the LC-MS/MS method were comparable to target values. Subsequently, this method has been applied to the therapeutic monitoring of digoxin in a clinical setting.
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