Generation of functional ventricular heart muscle from mouse ventricular progenitor cells. Science (New York, N.Y.) [Science] Journal article

Title	Generation of functional ventricular heart muscle from mouse ventricular progenitor cells.
Author(s)	Domian IJ, Chiravuri M, van der Meer P, Feinberg AW, Shi X, Shao Y, Wu SM, Parker KK, Chien KR
Institution	Cardiovascular Research Center, Massachusetts General Hospital, Charles River Plaza, CPZN 3200, 185 Cambridge Street, Boston, MA 02114-2790, USA.
Source	Science 2009 Oct 16; 326(5951):426-9.
MeSH	Action Potentials Animals Cell Cycle Cell Differentiation Cell Line Cell Lineage Cells, Cultured Embryonic Stem Cells Gene Expression Heart Heart Ventricles Mice Mice, Transgenic Muscle Development Myocardial Contraction Myocytes, Cardiac Oligonucleotide Array Sequence Analysis Tissue Engineering Ventricular Function
Abstract	The mammalian heart is formed from distinct sets of first and second heart field (FHF and SHF, respectively) progenitors. Although multipotent progenitors have previously been shown to give rise to cardiomyocytes, smooth muscle, and endothelial cells, the mechanism governing the generation of large numbers of differentiated progeny remains poorly understood. We have employed a two-colored fluorescent reporter system to isolate FHF and SHF progenitors from developing mouse embryos and embryonic stem cells. Genome-wide profiling of coding and noncoding transcripts revealed distinct molecular signatures of these progenitor populations. We further identify a committed ventricular progenitor cell in the Islet 1 lineage that is capable of limited in vitro expansion, differentiation, and assembly into functional ventricular muscle tissue, representing a combination of tissue engineering and stem cell biology.
Language	eng
Pub Type(s)	Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't
PubMed ID	19833966

Advertise on this site.