Generation of medaka fish haploid embryonic stem cells. Science (New York, N.Y.) [Science] Journal article

Title	Generation of medaka fish haploid embryonic stem cells.
Author(s)	Yi M, Hong N, Hong Y
Institution	Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
Source	Science 2009 Oct 16; 326(5951):430-3.
MeSH	Animals Cell Differentiation Cell Line Cell Proliferation Cell Shape Chromosomal Instability Cloning, Organism Crosses, Genetic Diploidy Embryo, Nonmammalian Embryonic Stem Cells Female Haploidy Male Nuclear Transfer Techniques Oocytes Oryzias Phenotype Pluripotent Stem Cells Transplantation Chimera
Abstract	Haploid embryonic stem (ES) cells combine haploidy and pluripotency, enabling direct genetic analyses of recessive phenotypes in vertebrate cells. Haploid cells have been elusive for culture, due to their inferior growth and genomic instability. Here, we generated gynogenetic medaka embryos and obtained three haploid ES cell lines that retained pluripotency and competitive growth. Upon nuclear transfer into unfertilized oocytes, the haploid ES cells, even after genetic engineering, generated viable offspring capable of germline transmission. Hence, haploid medaka ES cells stably maintain normal growth, pluripotency, and genomic integrity. Mosaic oocytes created by combining a mitotic nucleus and a meiotic nucleus can generate fertile fish offspring. Haploid ES cells may offer a yeast-like system for analyzing recessive phenotypes in numerous cell lineages of vertebrates in vitro.
Language	eng
Pub Type(s)	Journal Article Research Support, Non-U.S. Gov't
PubMed ID	19833967

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