Wang Q, Wang X, Liu H, Rose P, Zhu Y
Protective effects of cysteine analogues on acute myocardial ischemia: Novel modulators of endogenous H2S production. [JOURNAL ARTICLE]
Antioxid Redox Signal 2009 Oct 20.
The current study was designed to evaluate the pharmacological effects of three novel cysteine containing compounds namely, S-propyl-L-cysteine (SPC), S-allyl-L-cysteine (SAC) and S-propargyl-L-cysteine (SPRC) on HB2BS production and antioxidant defenses in an acute myocardial infarction (MI) rat model. The enzymatic activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), as well as glutathione redox status and malonaldehyde (MDA) content were also determined. All three compounds were found to preserve SOD and GPx activities and also tissue GSH levels whilst reducing the formation of the lipid peroxidation product MDA in ventricular tissues. Using immunfluorescence assays, we observed the expression of CSE and Mn-SOD. The morphologic changes of the cardiac cells are seen at both light and electron microscopy. The corresponding pathological alterations were mainly characterized as loss of adherence between cardiac myocytes and swollen or ruptured mitochondria at the ultrastrutural level. Propargylglycine, a selective inhibitor of CSE, abolished the protective effects of each compound used in the current model. Our study provides novel evidence that SPC, SAC and SPRC have cardioprotective effects in MI by reducing the deleterious effects of oxidative stress by modulating the endogenous levels of HB2BS and preserving the activities of antioxidant defensive enzymes like SOD.
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