| Title | Rapid Induction of P-Glycoprotein mRNA and Protein Expression by Cytarabine in HL-60 Cells. | | Author(s) | Prenkert M, Uggla B, Tina E, Tidefelt U, Strid H | | Institution | Clinical Research Centre, Orebro University Hospital, S-701 85 Orebro, Sweden. malin.prenkert@orebroll.se. | | Source | Anticancer Res 2009 Oct; 29(10):4071-6. | | Abstract | BACKGROUND: Overexpression of P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and glutathione-S-transferase pi (GSTpi) is associated with drug resistance in acute myeloid leukemia (AML). The short-term effects of drug exposure on their expression levels were investigated.
MATERIALS AND METHODS: HL-60 cells and drug-resistant sublines were cultured with or without daunorubicin (DNR) and cytarabine (Ara-C). At several time-points the expression levels of P-gp, BCRP and GSTpi were determined.
RESULTS: After exposure to Ara-C, P-gp mRNA rapidly increased in all the cell lines. P-gp protein was detected in the sensitive cells after 8 h exposure to Ara-C. GSTpi mRNA increased in the resistant cells, but no change in BCRP mRNA was observed. Exposure to DNR revealed rapidly increased P-gp and GSTpi mRNA in the resistant cells.
CONCLUSION: Ara-C rapidly increases P-gp mRNA and protein expression in sensitive and resistant cells, and GSTpi mRNA in resistant cells, in vitro. This may be of clinical importance during AML induction chemotherapy. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19846953 |
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