| Title | Teladorsagia circumcincta: survival of adults in vitro is enhanced by the presence of a mammalian cell line. | | Author(s) | Luque A, Walker LR, Pedley JC, Pedley KC, Hillrichs K, Simpson HV, Simcock DC | | Institution | Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11222, Palmerston North, New Zealand. | | Source | Exp Parasitol 2009 Oct 20. | | Abstract | Adult Teladorsagia circumcincta survival and motility in vitro was examined in a range of different cell culture media, supplements and gas mixes. Under optimum conditions, worms survived for 14 days, exhibiting high motility for 9 days and egg production for 72 hours. Optimum conditions involved co-culture of worms with a HeLa cell line in a supplemented cell medium (CEM) and an atmosphere containing 10% CO(2), 5% O(2) 85% N(2), 65% humidity at 37 degrees C. The incubation medium consisted of Minimum Essential Medium with 10% fetal calf serum, 1% non-essential amino acids, 1% glutamax and 1% penicillin-neomycin-streptomycin cocktail mix. Compared with optimum conditions, incubation in CEM alone, cell conditioned CEM, RPMI alone, Medium 199 alone, reduced CO(2) or O(2), or when cells were replaced with Escherichia coli, both survival and motility were reduced. Optimum conditions for adult T. circumcincta maintenance for culture, anthelmintic testing or generation of excretory/secretory products are described. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19852958 |
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