Identification of sildenafil, tadalafil and vardenafil by gas chromatography-mass spectrometry on short capillary column. Journal of chromatography. A [J Chromatogr A] Journal article | | Title | Identification of sildenafil, tadalafil and vardenafil by gas chromatography-mass spectrometry on short capillary column. | | Author(s) | Man CN, Nor NM, Lajis R, Harn GL | | Institution | National Poison Centre, Universiti Sains Malaysia, 11800 Penang, Malaysia. | | Source | J Chromatogr A 2009 Oct 12. | | Abstract | Sildenafil and its analogues (tadalafil and vardenafil) are phosphodiesterase type 5 inhibitors used in the treatment of male erectile dysfunction. Some dietary supplements, herbal preparations and food products which claim to enhance male sexual function have been found to be adulterated with these drugs. In this study, a gas chromatograph-mass spectrometer (GC-MS) assay was developed for identification of the drugs. In addition to good and short chromatographic separation that can be achieved within 6min by using a short 10m capillary column, no prior sample clean-up before GC-MS analysis was required, thus making this assay a cost saving and rapid method. Furthermore, the assay is specific as the identification of sildenafil, tadalafil and vardenafil were done by detection of molecular ions; m/z 474, 389 and 448, respectively, and several other characteristic ions resulted from the mass fragmentation of individual molecules. Using our currently developed assay, sildenafil and its analogues were successfully identified in food and herbal matrices. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19853256 |
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