A single LC-tandem mass spectrometry method for the simultaneous determination of four H(2) antagonists in human plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | A single LC-tandem mass spectrometry method for the simultaneous determination of four H(2) antagonists in human plasma. | | Author(s) | Sun X, Tian Y, Zhang Z, Chen Y | | Institution | Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China; Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, China. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2009 Oct 8. | | Abstract | A sensitive and universal LC-MS/MS method for the simultaneous determination of famotidine, cimetidine, ranitidine and lafutidine in human plasma was presented. This is the first single LC-MS/MS method reported for the simultaneous analysis of these four H(2) antagonists in human plasma. Following liquid-liquid extraction with ethyl acetate, the separation was performed on an Agilent Zorbax SB-CN (150mmx2.1mm I.D., 5mum) column using a mobile phase consisted of methanol:20mM ammonium acetate (55:45, v/v). The total run time was 7min per sample. Quantification was performed by electrospray ionization-triple quadrupole mass spectrometry by selected reaction monitoring (SRM) detection in the positive mode. All calibration curves showed good linear regression (r(2)>0.99) from 0.5 to 1000ng/mL for famotidine and lafutidine, and 5-20,000ng/mL for cimetidine and ranitidine. The method showed good precision and accuracy with overall intra- and inter-day variations of 1.37-9.29% and 3.51-9.40%, respectively. The assay was successfully applied to a bioequivalence study using ranitidine as the model compound. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19853541 |
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