Rambla-Alegre M, Esteve-Romero J, Carda-Broch S
Validation of a MLC method with fluorescence detection for the determination of quinolones in urine samples by direct injection. [JOURNAL ARTICLE]
J Chromatogr B Analyt Technol Biomed Life Sci 2009 Oct 9.
A sensitive and robust method was developed and validated for the routine identification and quantification of five quinolones in urine samples directly injected into a micellar liquid chromatographic system without any pre-treatment step. Since the simultaneous elution of the five compounds was not resolved, two mobile phases have been proposed: (a) for ciprofloxacin and levofloxacin 0.15M sodium dodecyl sulphate, 12.5% propanol and 0.5% triethylamine at pH 3.0 as the mobile phase and the detector at excitation wavelength 285nm and emission wavelength 465nm; and (b) for lomefloxacin, ofloxacin and moxifloxacin 0.05M sodium dodecyl sulphate, 12.5% propanol and 0.5% triethylamine at pH 3.0 as the mobile phase and the detector at excitation wavelength 295nm and emission wavelength 485nm. Using these conditions, and in accordance with the food and drug analysis (FDA) guideline, the limit of quantification was 1ng/mL, and the relative standard deviation and accuracy of the inter-day assay were 1.0-8.4% and 0.11-1.5%, respectively. Detection of the urinary excretion of four quinolones was followed up at 12h after the healthy volunteers had taken the drug. No potential interference from metabolites was observed. This procedure permits the rapid and reproducible measurement of low levels of quinolones in a small amount of urine.
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