Simultaneous Quantification of Erlotinib, Gefitinib, and Imatinib in Human Plasma by Liquid Chromatography Tandem Mass Spectrometry. Therapeutic drug monitoring [Ther Drug Monit] Journal article | | Title | Simultaneous Quantification of Erlotinib, Gefitinib, and Imatinib in Human Plasma by Liquid Chromatography Tandem Mass Spectrometry. | | Author(s) | Chahbouni A, den Burger JC, Vos RM, Sinjewel A, Wilhelm AJ | | Institution | From the VU University Medical Center, Department of Clinical Pharmacology and Pharmacy, Amsterdam, The Netherlands. | | Source | Ther Drug Monit 2009 Oct 27. | | Abstract | A quantitative liquid chromatography (LC)-mass spectrometry (MS)/MS method in human plasma was developed and validated for the tyrosine kinase inhibitors erlotinib, gefitinib, and imatinib in human plasma. Pre-treatment of the samples was achieved by using liquid-liquid extraction using D-8 imatinib as internal standard. Separation was performed on a Waters Alliance 2795 LC system using an XBridge RP18 column. The mass spectrometer Micromass was equipped with an electro spray ionization probe, operating in the positive mode. The calibration curves in plasma were linear for erlotinib, gefitinib, and imatinib over the concentration range of 5 to 3,000; 5 to 3,000, and 5 to 5,000 ng/mL, respectively. The intraday and interday accuracy ranged from 90% to 110% and the intraday and interday precision of the method was within 5%. The reported method provided the necessary linearity, precision, and accuracy to determine tyrosine kinase inhibitors in clinical research and for therapeutic drug monitoring. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19865001 |
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