| Title | Effects of Insulin-Like Growth Factor-1 on Rotenone-Induced Apoptosis in Human Lymphocyte Cells. | | Author(s) | Avila-Gomez IC, Velez-Pardo C, Jimenez-Del-Rio M | | Institution | School of Medicine, Medical Research Institute, Neuroscience Research Program, University of Antioquia, Medellin, Colombia. | | Source | Basic Clin Pharmacol Toxicol 2009 Oct 28. | | Abstract | Human peripheral blood lymphocytes have been useful as a putative model of oxidative stress-induced apoptosis for Parkinson's disease. The present work shows that rotenone, a mitochondrial complex I inhibitor, induced time- and concentration-dependent apoptosis in lymphocytes which was mediated by anion superoxide radicals (O(2)*(-))/hydrogen peroxide, depolarization of mitochondria, caspase-3 activation, concomitantly with the nuclear translocation of transcription factors such as NF-kappaB, p53, c-Jun and nuclei fragmentation. Since insulin-like growth factor-1 (IGF-1) interferes with a cell's apoptotic machinery when subjected to several stressful conditions, it is demonstrated here for the first time that IGF-1 effectively protects lymphocytes against rotenone through PI-3K/Akt activation, down-regulation of p53 and maintenance of mitochondrial membrane potential independently of ROS generation. These data might contribute to understanding the role played by IGF-1 against oxidative stress stimuli. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19874289 |
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