| Title | Determination of beta-hydroxybutyrate in blood and urine using gas chromatography- mass spectrometry. | | Author(s) | Hassan HM, Cooper GA | | Institution | Forensic Medicine and Science, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom. Huda.Hassan@formed.gla.ac.uk | | Source | J Anal Toxicol 2009 Oct; 33(8):502-7. | | Abstract | Beta-hydroxybutyrate (BHB) is considered a potential biomarker for alcoholic ketoacidosis (AKA). A robust and sensitive method was developed and validated for the quantitative determination of BHB in postmortem blood and urine using deuterated gamma-hydroxybutyrate as an internal standard. Samples were analyzed by gas chromatography-mass spectrometry following liquid-liquid extraction and silyl derivatization. The limits of detection and lower limits of quantification in blood and urine were 2 and 7 mg/L and 2 and 6 mg/L, respectively. The interday and intraday precision was measured by coefficients of variation for blood and urine and ranged from 1.0 to 12.4% for quality control samples spiked at 50 and 300 mg/L. The linear range of 50-500 mg/L resulted in an average correlation of R(2) > 0.99, and the average extraction recoveries in blood and urine were >or= 82% and >or= 59%, respectively. BHB remains stable in blood spiked at a concentration of 300 mg/L for 15 days when stored within a refrigerator (2-5 degrees C). Postmortem blood and urine samples were analyzed using the validated method for cases where the deceased had a history of chronic alcohol abuse to establish the use of BHB as a potential marker of AKA. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19874659 |
|
