| Title | Isolation and characterization of a novel short-chain alcohol dehydrogenase gene from Panax ginseng. | | Author(s) | Kim YJ, Shim JS, Lee JH, Jung DY, Sun H, In JG, Yang DC | | Institution | Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Suwon 449-701, Korea deokchunyang@yahoo.co.kr. | | Source | BMB Rep 2009 Oct 31; 42(10):673-8. | | Abstract | The cDNA of alcohol dehydrogenase (PgADH) was isolated and characterized from the leaf of Panax ginseng. The cDNA had an open reading frame of 801 bp and a deduced amino acid sequence of 266 residues. The calculated molecular mass of the mature protein is approximately 29 kDa with a predicated isoelectric point of 6.84. Homology analysis revealed that the deduced amino acid of PgADH shares a high degree of homology with the short-chain ADH proteins of other plants. Genomic DNA hybridization analysis indicated that PgADH represents a multi-gene family. The expression of PgADH under various environmental stresses was analyzed at different time points using real-time PCR. ABA, SA and especially JA (80-fold) significantly induced PgADH expression within 24 h of treatment. The positive responses of PgADH to abiotic stimuli suggest that ginseng ADH may protect against hormone-related environmental stresses. [BMB reports 2009; 42(10): 673-678]. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19874713 |
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