Zhou G, Tan ZR, Ouyang DS, Chen Y, Guo D, Xie HT, Liu YZ, Fan L, Deng HW
Development and Validation of a Simple and Sensitive LC-MS-MS Method for Quantifying Trimetazidine in Human Plasma. [JOURNAL ARTICLE]
Clin Exp Pharmacol Physiol 2009 Oct 29.
Abstract 1. A simple and sensitive liquid chromatography-tandem mass spectrometric method (LC-MS-MS) for quantifying trimetazidine in human plasma was developed and validated. Sample preparation was based on deproteinating with acetonitrile. 2. Chromatography was performed on a C-18 analytical column(5 mum, 150 x 2.1 mm i.d.) and the retention times were 1.8 and 3.0 min for trimetazidine and cetirizine(internal standard), respectively. The ionization was optimized using ESI (+) and enhanced selectivity was achieved using tandem mass spectrometric analysis. The calibration curve ranged from 0.1 to 200 ng mL(-1). The inter-day precision, accuracy and the relative standard deviation (RSD) were < 15%. The analyte was shown to be stable over the timescale of the whole procedure. 3. The robustness of the method was demonstrated by the good reproducibility of the results obtained during the analysis of clinical samples.
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