Chen XY, Zhuang YL, Li L, Zhang WW, Huang LL
The effect of mifepristone on the peripheral blood natural killer cells cytotoxicity and expression of CD94/NKG2A and NKG2D during the implantation phase. [JOURNAL ARTICLE]
Fertil Steril 2009 Oct 30.
OBJECTIVE: To investigate the effect of mifepristone on peripheral blood natural killer cells (pbNK) cytotoxicity and the expression of the inhibitory receptor CD94/NKG2A and the activated receptor NKG2D on pbNK cells.
DESIGN: In vitro study.
SETTING: University hospital and research laboratory.
PATIENT(S): Twenty healthy nonpregnant women.
INTERVENTION(S): Detected the cytolytic activity of pbNK to K562 target cells; measured the expression of CD94/NKG2A and NKG2D on pbNK.
MAIN OUTCOME MEASURE(S): Cytotoxicity of pbNK was detected by Methyl thiazolyl tetrazolium. The expression of CD94/NKG2A and NKG2D receptor on pbNK cells were detected by flow cytometry.
RESULT(S): The NK cell cytotoxicity and the expression of inhibitory receptor CD94/NKG2A during the proliferative phase (81.71 +/- 11.5, 86.6 +/- 9.0) was significantly higher than the secretory phase (60.16 +/- 19.2, 60.15 +/- 31.0). The NK cells cytotoxicity, after being treated with mifepristone and the expression of inhibitory receptor CD94/NKG2A on pbNK cells treated with 200 nmol/L mifepristone, were significantly increased. Mifepristone had no effect on the expression of activating receptor NKG2D.
CONCLUSION(S): These data suggest that Mifepristone maybe exert its anti-implantation function by increasing NK cytotoxicity. The increasing NK cytotoxicity of mifepristone is not related to CD94/NKG2A and NKG2D. In the secretory phase down-regulated CD94/NKG2A, NKG2D, and NK cytotoxicity may benefit with embryo implantation.
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