Extraction of chondroitin/dermatan sulfate glycosaminoglycans from connective tissue for mass spectrometric analysis. Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] Journal article | | Title | Extraction of chondroitin/dermatan sulfate glycosaminoglycans from connective tissue for mass spectrometric analysis. | | Author(s) | Bielik AM, Zaia J | | Institution | Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA. | | Source | Methods Mol Biol 2010.:215-25. | | Abstract | Chondroitin/dermatan sulfate (CS/DS) glycosaminoglycans (GAGs) are present in high levels in connective tissue where they play roles as structural molecules and in protein-binding interactions. Recent developments in the techniques for analysis of CS/DS using capillary electrophoresis (CE) have enabled progress in the understanding of changes in CS/DS structure that accompany connective tissue diseases including osteoarthritis. Key to these developments is the ability to extract CS/DS GAGs from small quantities of connective tissue. This chapter describes a method for connective tissue GAG extraction, derivatization, and workup for subsequent capillary electrophoretic and/or mass spectrometric analysis. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19882131 |
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