| Title | Pharmacodynamics of cisplatin-loaded PLGA nanoparticles administered to tumor bearing mice. | | Author(s) | Moreno D, Zalba S, Navarro I, de Ilarduya CT, Garrido MJ | | Institution | Department of Pharmacy and Pharmaceutical Technology. University of Navarra. Pamplona. Spain. | | Source | Eur J Pharm Biopharm 2009 Oct 30. | | Abstract | Biodegradable poly (lactic-co-glycolic) acid (PLGA) nanoparticles incorporating cisplatin have been developed to evaluate its in vivo efficacy in tumor bearing mice In vitro study proved two mechanism of action for cisplatin depending on the dose and the rate at which this dose is delivered. In vivo study, 5 mg/kg of cisplatin-nanoparticles administered to mice, exhibited a tumor inhibition similar to free-cisplatin, although the area under cisplatin concentration-time curve between 0 and 21 days (AUC(0-21)) had lower value for the formulation than for drug solution (P<0.05). This result was associated with a higher activation of apoptosis in tumor, mediated by caspase-3, after nanoparticles administration. Toxicity measured as the change in body weight and BUN (Blood Urea Nitrogen) plasma levels showed that cisplatin-nanoparticles treatment did not induce significant changes in both parameters compared to control, while for free drug a statistical (P<0.01) decrease was observed. In addition, a good correlation was found between time profiles of tumor volume and VEGF (Vascular Endothelial Growth Factor) plasma levels, suggesting that its expression could help to follow the efficacy of the treatment. Therefore, the PLGA nanoparticles seem to provide a promising carrier for cisplatin administration avoiding its side effects without a reduction of the efficacy, which was consistent with a higher activation of apoptosis than free-drug. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19883755 |
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