| Title | Role of T-type Ca Channels in the Spontaneous Phasic Contraction of Pregnant Rat Uterine Smooth Muscle. | | Author(s) | Lee SE, Ahn DS, Lee YH | | Institution | Department of Physiology, BK 21 Project for Medical Sciences, College of Medicine, Yonsei University, Seoul 120-752, Korea. | | Source | Korean J Physiol Pharmacol 2009 Jun; 13(3):241-249. | | Abstract | Although extracellular Ca(2+) entry through the voltage-dependent Ca(2+) channels plays an important role in the spontaneous phasic contractions of the pregnant rat myometrium, the role of the T-type Ca(2+) channels has yet to be fully identified. The aim of this study was to investigate the role of the T-type Ca(2+) channel in the spontaneous phasic contractions of the rat myometrium. Spontaneous phasic contractions and [Ca(2+)](i) were measured simultaneously in the longitudinal strips of female Sprague-Dawley rats late in their pregnancy (on day 18~20 of gestation: term=22 days). The expression of T-type Ca(2+) channel mRNAs or protein levels was measured. Cumulative addition of low concentrations (<1 microM) of nifedipine, a L-type Ca(2+) channel blocker, produced a decrease in the amplitude of the spontaneous Ca(2+) transients and contractions with no significant change in frequency. The mRNAs and proteins encoding two subunits (alpha1G, alpha1H) of the T-type Ca(2+) channels were expressed in longitudinal muscle layer of rat myometrium. Cumulative addition of mibefradil, NNC 55-0396 or nickel induced a concentration-dependent inhibition of the amplitude and frequency of the spontaneous Ca(2+) transients and contractions. Mibefradil, NNC 55-0396 or nickel also attenuated the slope of rising phase of spontaneous Ca(2+) transients consistent with the reduction of the frequency. It is concluded that T-type Ca(2+) channels are expressed in the pregnant rat myometrium and may play a key role for the regulation of the frequency of spontaneous phasic contractions. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19885043 |
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