| Title | The adenoviral E1A oncoprotein activates the Smad7 promoter: Requirement of a functional E-box. | | Author(s) | Dole VS, Smola H, Dooley S, Said HM, Oldak M, Pfister HJ, Smola S | | Institution | Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany. | | Source | Int J Oncol 2009 Dec; 35(6):1493-1498. | | Abstract | DNA tumorviruses like adenoviruses (AdV) or human papillomaviruses (HPV) have adopted various strategies to interfere with antiproliferative transforming growth factor-beta (TGF-beta) signalling. Here we report that the AdV E1A oncoprotein is sufficient to induce Smad7 expression, an inhibitor of TGF-beta signalling. E1A but not HPV oncoproteins activated the Smad7 promoter. A promoter proximal E-box was crucial for E1A-mediated transcriptional activity. E1A but not HPV oncoproteins induced specific binding activity at this E-box, which was identified as upstream stimulatory factor. In conclusion, these results unravel a novel mechanism of how the AdV E1A oncoprotein induces a cellular inhibitor of TGF-beta signalling. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19885573 |
|
